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Abstract The main research goal of this study is to decipher the intercorrelation between process-induced thermal-structure-property relationships of Stainless Steel 316L fabricated by laser powder bed fusion. The objective therein is achieved by explaining and quantifying the effect of processing parameters and part-scale thermal history on microstructure evolution and mechanical properties of these parts. Multiple previous works have correlated the effect of process parameters on flaw formation, microstructural features evolved and functional properties; however, a lack of understanding remains in the underlying effect of the thermal history on part microstructure and mechanical properties. The thermal distribution, or thermal history, of the part as it is being built layer-by-layer is influenced by the processing parameters, material properties and shape of the part. The thermal history influences the microstructure by changing the grain structure evolution, which affects the part properties. Therefore, the novelty of this paper lies in illuminating the process-thermal history-microstructure-property relationship in laser powder bed fusion. Characterization of tensile specimens processed at a variety of conditions reveal a direct influence of the choice of process parameters on the dendritic structure and the grain orientations. A high energy density leads to <100> textured columnar dendritic grains and low energy density leads to randomly oriented equiaxed grains as a result of the shifting heat influx. The tensile properties are correlated with the inherent microstructure. Through future work involving fracture surface analysis, the texture, grain size and porosity is expected to influence the inherent fracture mechanism. This work demonstrates that an understanding of thermal distribution within a printed part can inform the choice of processing conditions to generate the final microstructure as per the specified functional requirements. Thus, this paper lays the foundation for future prediction and control of microstructure and functional properties in laser powder bed fusion by identifying the root fundamental thermal phenomena that influences the microstructure evolution and part properties.